Cytokine Responses of TNF-α, IL-6, and IL-10 in G6PD-Deficient Infants

G6PD-deficient adults are reported to be susceptible to severe infection, and decreased cytokine responses have been postulated as the underlying mechanism. However, investigating the association of G6PD deficiency and cytokine responses during infancy is lacking. The current study aims to determine whether cytokine responses of tumor necrosis factor ()-α, interleukins (IL)-6, and IL-10 are impaired in the G6PD-deficient infants. Upon agreements with informed consents, peripheral blood mononuclear cells (PBMCs) of enrolled infants were collected twice at 1 month and 1 year of age. PBMCs were then stimulated with toll-like receptor (TLR) agonists—including PAM3csk4 for TLR1–2, poly (I:C) for TLR3, and lipopolysaccharide for TLR4—to analyze the expression of TNF-α, IL-6, and IL-10. Males (P = .004) and phototherapy during neonatal period (P = .008) were more common among G6PD-deficient infants than G6PD-normal subjects. After the stimulation of TLR agonists, there was no significant difference in the expression of TNF-α, IL-6, and IL-10 between PBMCs of G6PD-deficient and -normal infants at both 1 month and 1 year of age. In conclusion, the clinical characteristics of G6PD-deficient infants are different from those of G6PD-normal subjects. The data suggest that the innate immune responses to TLR agonists in G6PD-deficienct infants are not different from those of G6PD-normal infants.     

川芎嗪对离体豚鼠气道平滑肌的效应及机制研究

目的：探讨川芎嗪( ligustrazine,LIG)对离体豚鼠气道平滑肌( airway smooth muscle,ASM)的效应及机制。方法取正常豚鼠支气管,制作成气管螺旋条,随机分成LIG组、LIG+普萘洛尔组、LIG+阿托品组、LIG+硝苯地平组、LIG+格列苯脲组、对照组。分别观察ASM在静息张力下以及组胺所致的高张力下,各组不同LIG浓度对ASM的效应并比较各组间ASM舒张率。结果(1) LIG对离体豚鼠ASM静息张力以及组胺所致的高张力均具有舒张作用。(2)离体豚鼠ASM在静息张力下,与LIG组比较,LIG+普萘洛尔组在LIG浓度10-7~10-6．5 mol/L时,舒张作用明显受到拮抗( P <0．05)。 LIG +阿托品组、LIG+格列苯脲组在LIG浓度10-7．5~10-3 mol/L时,舒张作用没有明显差别(P>0．05)。 LIG+硝苯地平组在LIG浓度10-6~10-4 mol/L时,舒张作用明显加强( P<0．05)。(3)离体豚鼠ASM在组胺所致的高张力下,与LIG组比较,LIG+普萘洛尔组在LIG浓度10-6.5~10-5 mol/L时,舒张作用明显受到拮抗( P<0．05);LIG+阿托品组、LIG+格列苯脲组在LIG浓度10-8~10-3 mol/L时,舒张作用没有明显差别( P>0．05);LIG+硝苯地平组在LIG浓度10-6.5~10-5 mol/L时,舒张作用明显加强(P<0．05)。结论 LIG可对离体豚鼠ASM静息张力以及组胺所致的高张力产生明显的舒张作用,其效应的产生可能与竞争性激动β2受体以及抑制Ca2+内流有关,这为临床应用川芎嗪治疗支气管哮喘提供了理论依据。     

早产儿视网膜病的高危因素分析

目的了解我院早产儿视网膜病（refinopathy of prematurity,ROP）的发病状况,并对其高危因素进行分析。方法对2010年1月至2012年12月在我院新生儿科住院的早产儿（胎龄≤36周,体重≤2．5kg）,于生后2周进行ROP筛查,并定期随访。将患儿全身状况及吸氧、母孕期吸氧、先兆子痫、胎盘早剥等因素进行分析。结果255例患儿全部完成了眼底筛查,在周边视网膜血管化或病变退化后终止随访,发现ROP16例（26只眼）,ROP患病率为6．3％（5．1％）,其中Ⅰ期12例,Ⅱ期3例,Ⅲ期1例。高危因素分析示胎龄、出生体重、吸氧时间,吸氧浓度、机械通气与ROP相关（P〈0．05）;母孕期吸氧、先兆子痫、胎盘早剥等因素与ROP发病无关。结论早产、吸氧浓度高、机械通气是ROP的主要危险因素。对早产儿适时进行ROP筛查,并对发现的ROP早期进行有效视网膜激光光凝术,可控制病变,降低早产儿的致盲率。     

反复烧烤对渗透陶瓷-面瓷复合体颜色的影响

目的 :探讨反复烧烤后渗透陶瓷 饰面瓷复合体的颜色变化规律。方法 :制作直径为 12 .5mm ,厚度为 0 .5mm的VitaIn CeramAlumina和GI Ⅱ型渗透陶瓷片状样本 ,表面烧结 0 .2mm的Vitaduralpha不透明牙本质瓷 ,0 .6mm的牙本质瓷 ,0 .2mm的牙釉质瓷 ,表面打磨后 ,按饰面瓷烧结程序反复烧烤 2、4、6、8、10次 ,用TC PⅡG全自动测色色差仪测量不同烧烤次数以后样品的颜色参数 ( 1976CIEL a b ) ,计算色相值 (hab)、色饱和度 (C ab)参数和色差值 (ΔE)。结果 :反复烧烤以后 ,GI Ⅱ样本的明度值降低 (P <0 .0 5 ) ,色相值和彩度无明显变化 (P >0 .0 5 ) ;VitaIn CeramAlumina样本颜色变化不明显 (P >0 .0 5 )。 10次反复烧烤所引起的颜色变化 (ΔE)最大值为 2 .2 798(GI Ⅱ样本 )和 1.3894(VitaIn CeramAlumina样本 )。两种材料样本 10次以后的色差值最大为 2 .2 95 6。结论 :VitaIn CeramAlumina和GI Ⅱ型渗透陶瓷 饰面瓷复合体在反复烧烤以后 ,具有较好的颜色稳定性 ,10次反复烧烤以后颜色的变化程度处于临床可接受的范围之内     

牵引弓联合前牵引矫治替牙期前牙反(牙合)的牙颌结构变化

目的探讨牵引弓联合前方牵引对矫治安氏Ⅲ类错     

Long-term effects of lip repair on dentofacial morphology in patients with unilateral cleft lip and palate.

OBJECTIVE: Lateral cephalograms from the growth archive of the Sri Lankan Cleft Lip and Palate Project were analyzed in a cohort design to study the long-term effects of lip repair on dentofacial morphology in patients with unilateral cleft lip and palate. METHODS: A total of 71 patients were recruited, including 23 adult patients with nonsyndromic unilateral cleft lip and palate without surgical repair and 48 adult patients with nonsyndromic unilateral cleft lip and palate who had lip repair, but without management of alveolus or anterior vomer. The design utilized exact matching on ethnicity and statistical control for gender and age. RESULTS AND CONCLUSIONS: The data support the hypothesis that lip repair primarily produces a bone-bending effect on the anterior maxillary alveolus (alveolar molding), accompanied by controlled uprighting of maxillary incisors, and secondarily produces a bone-remodeling effect (bone resorption) in the base of the anterior maxillary alveolus. When analyzed by the age at lip repair and the surgeon who performed lip repair, early lip repair produced a greater bone-remodeling effect than did late lip repair, and variation in the surgeon who performed lip repair had an insignificant impact on dentofacial morphology after adjusting for covariates.     

磷酸钙水门汀的实验研制

作者在实验室合成了磷酸四钙,并研制出了磷酸钙水门汀。通过对磷酸钙水门汀凝固反应和主要性能的研究,结果表明磷酸钙水门汀有良好的理化性能,它凝固后的最终产物是羟基磷灰石。因此,磷酸钙水门汀是一种新型的、具有很大前途的粘接、垫底、根管充填材料。     

变链菌细胞外多糖合成与乳牙高龋的关系研究

目的:比较高龋和无龋幼儿变链菌临床分离株在体外利用蔗糖合成水溶性、水不溶性葡聚糖的能力。方法:蒽酮法定量测定从高龋(dmft≥5)和无龋幼儿(dmft=0)牙菌斑分离的变形链球菌临床分离株水溶性和水不溶性葡聚糖产量。结果:高龋组菌株合成水溶性与水不溶性葡聚糖量平均分别为0.0332mg/ml和0.0357mg/ml,高于无龋组菌株的0.0215mg/ml和0.0192mg/ml;高龋幼儿定植的合成水溶性及水不溶性葡聚糖能力强的菌株所占比例也高于无龋幼儿;变链菌临床菌株水不溶性葡聚糖产量平均为0.0301mg/ml,高于水溶性葡聚糖产量(0.0267mg/ml);差异有统计学意义。结论:高龋幼儿的龋活跃性与其口腔中变形链球菌临床菌株合成细胞外多糖能力强有关。